FastQCFastQC Report
Thu 8 Feb 2018
YD8_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62661480
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG113800.010.09388351
AGGGCCG92900.09.528121
AGGGGCG107950.09.3264271
TGGGGTT377350.09.0486791
TGGGCCG96000.08.8215921
TGGGGCC295400.08.5777311
GGGGTTT405100.08.5475942
AGGGGGG138950.08.5100321
AGGGTTT351350.08.5067041
AGGGGGC237850.08.3236941
TGGCCAG610150.08.29093551
TGGCCGG139500.08.2827311
TAGCCCG26800.08.2361011
TAGGCCG35600.08.2247711
TGGGGCT354500.08.1960371
TGGGGGC330650.08.1877831
AGGGGCC245250.08.1551951
TGGGCGT121900.08.129791
AGGGGCA286550.08.0644981
TAGCCAG349950.08.0001021
Produced by FastQC (version 0.11.5)