FastQCFastQC Report
Thu 8 Feb 2018
YD9_k27me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_k27me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57843272
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG98950.011.9213841
TGGGGTT311850.010.2073941
TAGGCCG25150.010.2041791
TGGCCGG72950.010.0292681
TCCCGTT112450.09.7694821
AGGGGCG46000.09.3473111
TAGCCGA17000.09.2695861
GGGGTTT337500.09.15980152
TAGGGCG10250.09.004741
AGGGTTT327000.08.956551
CCAACGA124250.08.8733639
GCGTGAG121000.08.8696423
GGCGTGA188400.08.8612732
TCCAACG127350.08.7456968
CGTGAGC117950.08.7368514
TGGGGCG42700.08.6989581
TGGGGCC185350.08.6355131
TGGCCAG494200.08.6139031
AGGCCGG92700.08.5724741
TAGCCAG335500.08.5082041
Produced by FastQC (version 0.11.5)