FastQCFastQC Report
Thu 8 Feb 2018
OD10_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD10_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58085876
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG37550.011.5827251
ACGGGGT176050.010.8932361
TAGCCGG171950.010.3273131
AGGCCGG191850.010.24278451
TGGGCCG93300.09.6855911
TGGCCGG150200.09.6322661
TAGCCCG23850.09.5432511
TGGGGTT411800.09.5110141
AGGGCCG96500.09.364411
AGGGCGG109650.09.2484131
GCGTGAG180000.09.2256163
AGGGGCG102700.09.1282231
AGGGTTT357300.09.0633151
CGGGGTT193150.09.0518612
CGTGAGC177700.08.9904724
TAGCCGA24400.08.9587051
GGCGTGA295550.08.9381712
TGGGGCG98150.08.9314641
AGGCGTG324200.08.8625871
AGGCGCC187800.08.8437261
Produced by FastQC (version 0.11.5)