FastQCFastQC Report
Thu 8 Feb 2018
OD11_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD11_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61271323
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA36200.011.31148612
TGGCCGG117500.010.7024751
ACGGGGT153250.010.5881451
TAGGCCG35500.010.5382291
AGGCCGG157100.010.2856291
TCCCGTT94500.010.2785851
TGGCGTT43550.010.1427521
CGTATAA40000.09.89941513
CTTCGTA48300.09.7820310
TGGGGCG80450.09.5524821
TAGCCGG135250.09.5478421
TGGGGTT396200.09.5333911
GCGTGAG187800.09.34573
AGGGCCG80350.09.3399871
AGGGGCG82600.09.2492711
ACTAGAC134300.09.24740622
AGGGCGG91000.09.1632221
CGTGAGC187150.09.1497174
TGGGCCG75950.09.1095831
ATCTTCG58850.09.0988678
Produced by FastQC (version 0.11.5)