FastQCFastQC Report
Thu 8 Feb 2018
OD12_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD12_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50612713
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG151700.010.933211
ACGGGGT144400.010.721241
TGGCCGG112050.09.9350611
AGGCCGG148650.09.6567281
TGGGGTT353350.09.4131881
TGGGCCG67800.09.1734961
GCGTGAG149450.09.06283953
CGTGAGC147800.08.844344
TAGGCCG31100.08.8400541
CGGGGTT155600.08.8058532
AGGGCCG68050.08.7755271
AGGCGCC132700.08.7456441
AGGGGCG75050.08.7377131
TGGCCAG634600.08.6680861
AGGGTTT315100.08.6606651
AGGGCGA43950.08.6140191
TAGCCAG396100.08.5508571
ACCGCGC52800.08.4796812
TAGCCGC13000.08.3205751
GGGGTTT410700.08.2994952
Produced by FastQC (version 0.11.5)