FastQCFastQC Report
Thu 8 Feb 2018
OD13_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD13_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66888267
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA79950.031.8211081
CTCGTAT81050.030.67734542
CGTATGC82300.030.37545244
TCGTATG84600.029.41665343
TCTCGTA84850.029.25042341
ATCGGAA87900.029.0556162
ATCTCGT96900.025.68249540
ACACGTC105700.024.41393913
TCGGAAG111600.023.1271613
CGGAAGA115300.022.911944
CGTCTGA113450.022.70651216
GTATGCC127800.020.05401845
ACGTCTG139000.018.96977815
CACGTCT147300.018.00779214
AATCTCG151450.017.39774139
CACACGT151500.017.37491212
GCACACG158800.016.67536711
GACCAAT169950.015.26361935
ACCAATC181000.014.41879136
ACTGACC212350.012.32187732
Produced by FastQC (version 0.11.5)