FastQCFastQC Report
Thu 8 Feb 2018
OD14_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD14_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59756696
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3331960.5575877220521027TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA378500.042.7311821
ATCGGAA389050.041.8107382
CTCGTAT385250.040.8672542
TCTCGTA386700.040.7314741
CGTATGC386550.040.7298144
TCGTATG388850.040.488943
TCGGAAG409250.039.5765953
ATCTCGT399500.039.43769540
ACACGTC411400.039.3423213
CGGAAGA414400.039.166194
CGTCTGA415600.038.6740616
GTATGCC423650.037.27954545
ACGTCTG440750.036.69695715
CACGTCT447800.036.17447714
CACACGT450900.036.00560812
GCACACG462400.035.12473711
GTCACAC528550.030.24774629
AGAGCAC550450.029.6738348
TCTGAAC545650.029.47711618
GTCTGAA547000.029.42904517
Produced by FastQC (version 0.11.5)