FastQCFastQC Report
Thu 8 Feb 2018
OD15_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD15_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62428237
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28250.013.167781
CTCGTAT31150.012.06339242
CGTATGC31300.011.86180144
TCTCGTA32600.011.80292141
CGCCAAT34200.011.31501833
ACGGGGT173200.011.1552691
TCGTATG35200.010.73934243
ATCGGAA34850.010.71668152
TATCTCG36400.010.57031639
TAGGCCG39000.010.2896941
TAGCCGG168600.010.2026751
TGGCCGG140550.09.4478191
CGGGGTT184750.09.279522
AGGCCGG182050.09.2259851
AGGGCCG90750.08.9434061
AGGGGCG94250.08.8744131
TGGGGTT412450.08.8167831
AGGCGCC172800.08.6239211
AGGCGTG316600.08.4952581
GCGTGAG178500.08.4196343
Produced by FastQC (version 0.11.5)