FastQCFastQC Report
Thu 8 Feb 2018
OD16_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD16_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35514633
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG97900.012.0579421
TGGGGTT255400.010.0836771
AGGCCGG105550.09.9223371
TGGCCGG82050.09.8482411
TAGGCCG19800.09.8036421
AGGGTTT225350.09.7155721
TGGGCCG50250.09.6124011
AGGGGCG57550.09.6089291
TGGCCAG454000.09.4560311
TGGGGCG54200.09.2450211
AGGGCCG53600.09.2221781
TAGCCCG14550.09.1525731
AGGCGCC95950.09.1507951
GGCGTGA168150.09.1250822
GCGTGAG103150.09.073473
TGGCGAA40550.09.0731
GGGGTTT289900.08.9248092
TGGGCGG72450.08.8477761
TAGCCGA13800.08.83221
CGTGAGC103250.08.7596224
Produced by FastQC (version 0.11.5)