FastQCFastQC Report
Thu 8 Feb 2018
OD17_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD17_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34857886
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG22400.013.6069731
TAGCCGG107700.013.2068911
TGGCCGG77700.010.954591
TGGGGTT242100.010.8271261
AGGGTTT225850.010.54649451
TGGCCAG462900.010.5108021
TGGGGCG42400.010.277021
AGGGCCG45900.010.1328191
TAGCCAG291900.010.0937411
TGGGCCG46650.09.92151451
GGGGTTT286800.09.8762812
TAGCCGA14750.09.643271
AGGCCGG99350.09.5445881
TAGCCCG13100.09.3067541
CGGGCGT63050.09.0991735
CGGCTAA70400.09.07595920
CCGGGCG111400.09.047734
TGGCAAA204450.08.9448621
ACCGCGC29450.08.93814612
TAGGCGT18650.08.8373041
Produced by FastQC (version 0.11.5)