FastQCFastQC Report
Thu 8 Feb 2018
OD18_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD18_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52074811
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG34450.011.3981151
TAGCCGG139250.011.1646661
TGGCCGG127650.011.034641
TGGGCCG79950.010.8221751
AGGCCGG166500.010.6948391
AGGGGCG88000.010.221331
TGGGGTT354950.010.0591991
ACGGGGT139400.09.7933721
TGGGGCG80500.09.7556531
TGGCCAG653100.09.7175981
TCCCGTT61050.09.4234231
AGGGTTT319200.09.4121061
AGGGCGG96350.09.3355161
TGGCCCG66650.09.2824571
GGCGTGA258700.09.0510012
TAGCCCG20750.09.0217261
GCGTGAG162400.08.9887853
GGGGTTT406100.08.861952
AGGGCCG80200.08.8527831
TGGGCGG116550.08.7556641
Produced by FastQC (version 0.11.5)