FastQCFastQC Report
Thu 8 Feb 2018
OD19_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD19_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52176695
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG144850.011.8637381
TAGGCCG32050.010.6390861
TGGCCGG114000.010.2013531
TGGGGTT353400.010.09592151
TAGCCGA19850.09.4421961
TGGGCCG70050.09.3163351
TAGCCCG20700.09.1635621
GGGGTTT405500.09.0657182
AGGCGCC126850.09.0611371
TGGGGCG70600.09.0518461
TGGCCAG624050.08.9523221
AGGCCGG144850.08.948471
AGGGTTT317150.08.935821
AGGGGCG70000.08.7745781
AGGGCCG69050.08.7317841
TAGCCAG384750.08.7215881
TGGCGAA55950.08.6371221
ACGGGGT131850.08.5805071
TGGGGGG209750.08.4189941
TGGGCCA256600.08.360311
Produced by FastQC (version 0.11.5)