FastQCFastQC Report
Thu 8 Feb 2018
OD20_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD20_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55169128
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG126700.011.6201541
TAGGCCG30850.010.8765451
TGGGCCG73950.010.6181551
TGGCCGG118150.010.3938711
AGGCCGG148800.010.2778121
TGGGGCG82700.09.8535221
TGGGGTT365850.09.7954721
TAGCCGA19750.09.5926761
AGGGCCG78150.09.5801791
AGGGGCG84900.09.1949041
AGGCGTG266850.09.1611731
GCGTGAG162050.09.1470153
AGGCGCC145050.09.09575751
GGCGTGA247900.09.0007382
AGGGTTT331950.08.9873511
ACGGGGT134100.08.9193121
CGTGAGC161200.08.9161814
TGGCCAG632000.08.9136761
AGGGCGG91450.08.885771
TGGCGTT37300.08.8733571
Produced by FastQC (version 0.11.5)