FastQCFastQC Report
Thu 8 Feb 2018
OD4_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60486885
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG177550.012.5372071
TAGGCCG39250.011.7218151
TGGCCGG134750.011.24721051
TAGCCGA22600.010.278581
TGGGCCG84550.09.9761361
TGGGGTT406450.09.9101141
TAGCCCG22250.09.6293721
AGGCCGG176150.09.5512571
AGGGTTT374800.09.52545451
TGGCCAG750150.09.3501081
AGGGGCG85800.09.2787991
AGGGCCG80650.09.0323861
TAGCCAG479700.09.0315441
GGGGTTT472450.08.9574972
TGGGGCG79800.08.9307621
TGGCCCG67500.08.8875531
CGGCTAA129750.08.68728520
CGGGCGT104900.08.5575845
AGGCGCG68950.08.4389761
AGGGCGG92750.08.3889881
Produced by FastQC (version 0.11.5)