FastQCFastQC Report
Thu 8 Feb 2018
OD5_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42413720
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22500.020.3487971
CTCGTAT26050.018.05180242
CGATGTA25900.017.98171434
CGTATGC26800.017.6301444
TCTCGTA27550.017.06894541
CCGATGT28250.016.80445733
ATCGGAA27600.016.792332
ACCGATG28500.016.57810832
TATCTCG29150.016.44059239
CACCGAT29200.016.2574531
TCGTATG30100.015.47334943
ATCTCGT35450.013.70941740
ACACGTC41000.011.85284813
TCGGAAG41500.011.8184693
GTCACCG41600.011.73597729
CGGAAGA45700.011.3723084
ACGGGGT104300.011.266231
TAGCCGG111150.011.2415311
TCACCGA42800.011.09153130
CGTCTGA45450.010.89034516
Produced by FastQC (version 0.11.5)