FastQCFastQC Report
Thu 8 Feb 2018
OD6_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD6_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50954027
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG33900.011.9043731
TAGCCGG152300.011.90171051
TGGCCGG116750.010.5436011
TGGGGTT343850.010.3530031
TGGGCCG72700.09.923591
GGGGTTT413200.09.42522052
AGGGTTT317000.09.2314231
TGGGGCG69050.09.1094831
AGGGCCG71700.09.0872381
TAGGCGC21250.09.0180621
TGGCCAG638900.08.9630141
AGGCCGG154100.08.9244291
AGGGCGG80800.08.8171641
ACGGGGT138800.08.7874131
AGGGGCG70850.08.6871241
GCGTGAG145850.08.6384613
TAGCCAG410700.08.5800041
TGGCCCG60250.08.53161051
AGGGCGA45150.08.3389611
AGGCGCC139900.08.2670941
Produced by FastQC (version 0.11.5)