FastQCFastQC Report
Thu 8 Feb 2018
OD7_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD7_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65057762
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG201950.013.6477391
TAGGCCG39950.010.78325751
TGGGGTT437300.010.6815421
TGGCCGG133600.010.4162661
TGGCCAG823850.010.2636231
TGGGCCG72850.010.0311061
TGGCGAA66650.09.8813451
CGGGCGT112750.09.6778185
GGGGTTT511800.09.6359052
TAGCCCG24500.09.5741591
AGGGTTT406450.09.4501971
TAGCCAG540750.09.2470361
CCGGGCG191550.09.2436794
AGGCCGG164550.09.0190621
AGGCGCC131950.08.9910351
TAGCCGA27700.08.9566641
GCCGGGC376800.08.9444413
AGGGGCG69450.08.8009681
CGAACTC242600.08.73599416
TGGCAAA394150.08.6750321
Produced by FastQC (version 0.11.5)