FastQCFastQC Report
Thu 8 Feb 2018
OD8_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD8_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43576335
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG109550.012.7752411
TGGCCGG101050.010.9951331
TAGGCCG28300.010.9896161
TGGCGAA49250.010.2044251
TGGGCCG66350.010.0880081
TGGGGCG70150.09.95918751
AGGGCCG64400.09.3786171
AGGCCGG131600.09.2989481
AGGGTTT276200.09.2692491
TAGCCAG297550.09.1797861
AGGGCGG80250.09.1550861
TGGCCAG526800.09.1421561
AGGGGCG72300.09.13312151
TGGGGTT305100.09.1151291
GGCGAAA47150.09.1141542
TAGCCGA17950.09.0397821
CGGGCGT71950.08.8808035
TAGCCCG17750.08.6337691
AGGGGGG104400.08.5915691
AGGGCGA40550.08.5033581
Produced by FastQC (version 0.11.5)