FastQCFastQC Report
Thu 8 Feb 2018
OD9_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD9_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41067472
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG109000.012.569111
TAGGCCG26050.012.3696031
TGGGGTT271850.010.5932431
AGGGCGG66550.010.2594011
TGGGCCG54300.010.2500171
TGGCCGG92950.09.9879251
AGGCCGG115350.09.6502091
AGGGTTT255200.09.6420511
TGGCCAG501100.09.5511911
TGGGGCG56750.09.5295591
AGGCGCC104800.09.4391011
TAGCCAG299150.09.3704151
AGGGGCG62000.09.3404731
GGGGTTT308500.09.3072582
TGGGCGG80100.09.0583861
AGGGCCG57250.09.0133741
TGGCAAA241300.08.9087851
TGGGGCA216300.08.8862791
AGGGGGG90650.08.8741711
TAGCCCG14250.08.8552461
Produced by FastQC (version 0.11.5)