FastQCFastQC Report
Thu 8 Feb 2018
YD10_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD10_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30717502
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG97650.011.5667731
TAGGCCG19150.09.7713841
TGGGCCG33650.09.7147071
ACGGGGT87100.09.6805551
TGGGGTT206600.09.4827921
TGGCGAA31600.09.2747681
TGGCCGG61000.08.9070531
AGGGGCG34750.08.69354251
TAGGGCG9400.08.6341871
AGGCCGG78450.08.6213461
TGGCAAA183800.08.4757731
TCCCGTT35800.08.3126151
TGGCCAG365900.08.29951
GCGGATC49450.08.2805728
AGGGTTT188350.08.2710231
TGCGAAA13650.08.2581761
CGGATCA51400.08.22905429
GGGGTTT246900.08.1648012
CGGGGTT93400.08.14195252
GGCGAAA30750.08.1215062
Produced by FastQC (version 0.11.5)