FastQCFastQC Report
Thu 8 Feb 2018
YD11_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD11_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52052133
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG175600.011.5884951
ACGGGGT156450.010.7599151
TGGGGTT355050.010.01571
TAGGCCG33200.09.6386031
TGGCCGG119300.09.067021
CGGGGTT172550.08.9980812
GGGGTTT435200.08.9241682
AGGCCGG151750.08.8507771
TGGGGCG64700.08.7076221
AGGGTTT324600.08.671171
TGCGACG6553.6379788E-128.6012691
TGGGCCG68450.08.5927241
TAGGCGT26500.08.5889351
TGGCCAG673600.08.5076211
TGGCGAA56550.08.4881061
TAGCCAG457800.08.45691
AGGGCCG64550.08.4136531
GCGTGAG139650.08.3303913
AGCCGGG277400.08.3187361
TGGCAAA309050.08.2470321