FastQCFastQC Report
Thu 8 Feb 2018
YD12_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD12_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47657137
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA26000.020.644761
CTCGTAT29700.017.57562642
TCTCGTA29900.017.53331441
ATCGGAA30300.017.5235922
CGTATGC30500.016.8929144
TCGTATG34700.014.91335543
ATCTCGT41750.012.61068440
ACACGTC43900.012.45361813
TCGGAAG46750.011.74253753
CGTCTGA47600.011.5328516
CGGAAGA51750.010.8253714
ACGGGGT126750.010.72940351
TAGCCGG123950.09.9892311
TGGCCGG98950.09.7095711
TGGGGTT313250.09.5108191
TAGCCCG17300.09.3862611
TAGGCCG26050.09.2636531
TGGGCCG57700.09.14631751
GTATGCC60550.09.14120645
AGGCCGG124950.09.0609481
Produced by FastQC (version 0.11.5)