FastQCFastQC Report
Thu 8 Feb 2018
YD14_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD14_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60919673
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA61250.031.9484441
CTCGTAT65750.028.91532742
TCTCGTA68500.027.8200741
CGTATGC69550.027.72367944
ATCGGAA72000.027.4666522
TCGTATG77150.024.87598443
TCACGAT79600.024.22239535
ATCTCGT79500.024.11225540
ACACGTC84300.023.6191913
TCGGAAG87400.022.7556973
CGTCTGA93150.021.08534416
CGGAAGA96500.020.912914
CATCACG96700.020.07861133
ACGTCTG109400.018.11790315
GTATGCC111000.017.89801645
CACGTCT117850.017.18154514
CACACGT126750.015.99286112
GCACACG128050.015.77778711
ACGATCT134000.015.32905237
ATCACGA129050.015.11507934
Produced by FastQC (version 0.11.5)