FastQCFastQC Report
Thu 8 Feb 2018
YD15_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD15_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50417263
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25000.018.8689171
CGATGTA28000.016.95406734
CTCGTAT29750.015.2767142
ATCGGAA33100.014.3418092
CGTATGC31900.014.31761744
TCTCGTA32350.014.11842641
CCGATGT33600.013.86055333
TATCTCG33450.013.85554439
CACCGAT33800.013.77853831
ACCGATG33250.013.73579632
TCGTATG35000.013.04948543
TAGCCGG124700.011.1856891
ATCTCGT42350.010.99718340
ACACGTC46100.010.73671113
GTCACCG47100.010.22215229
TCACCGA46500.010.208930
CGGAAGA54750.09.9033564
TGGCGAA46300.09.8471621
CGTCTGA50300.09.7507516
ACGGGGT129050.09.6718121
Produced by FastQC (version 0.11.5)