FastQCFastQC Report
Thu 8 Feb 2018
YD16_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD16_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62027739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA34300.023.8252581
CTCGTAT38500.021.0320942
TCTCGTA40150.020.1116741
CGTATGC40950.019.82868844
ATCGGAA43200.019.1605782
TCGTATG43550.018.85147743
ATCTCGT56300.014.7819840
ACACGTC58700.014.44576113
TCGGAAG64000.013.5660013
CGTCTGA64250.013.47797916
CGGAAGA69200.012.9041364
CATCTCG78550.011.25344839
ACGGGGT160750.011.1898421
TAGCCGG171900.010.9155251
GTATGCC81950.010.56701745
ACGTCTG86100.010.37109215
CACGTCT94150.09.53212514
TGGCGAA61900.09.2930571
CGGGGTT174400.09.2473482
TAGGCCG35650.09.156411
Produced by FastQC (version 0.11.5)