FastQCFastQC Report
Thu 8 Feb 2018
YD17_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD17_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52418084
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG36350.012.486351
TGGGCCG89700.011.6807151
TAGCCGG143400.010.6921361
TGGCCGG140150.010.6661761
AGGCCGG186600.010.0319821
ACGGGGT151350.010.026071
TGGGGTT368150.09.7341041
AGGGGCG95050.09.6691031
TGGGGCG93000.09.3480631
AGGCGCC179300.09.294371
AGGGCCG89950.09.2884751
TGGCCAG676650.09.28737451
GGCGTGA268400.09.1198532
AGGGTTT315600.09.1082571
AGGCGTG289100.08.9824251
TGGGGCC337650.08.9013361
TGGCCCG80050.08.885731
AGGGCGG108800.08.8414721
TGGCGAA69750.08.8381691
AGGCCCG85600.08.8372051