FastQCFastQC Report
Thu 8 Feb 2018
YD18_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD18_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47354879
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG134450.011.1535141
TAGGCCG31250.010.8404081
TGGGCCG68050.010.6864181
TGGGGTT324800.010.1795711
TGGCCGG112100.010.1538161
AGGCCGG142350.09.7095331
TAGCCGA20300.09.6789361
TAGGCGC17700.09.569571
AGGGGCG73700.09.5300971
TGGCCAG598800.09.3874471
AGGGTTT280700.09.3812471
TGGGGCG71750.09.3799111
TAGCCAG364750.09.1017821
GGGGTTT372650.09.0981272
TGGCGAA51350.09.0160811
AGGGCCG69950.08.9432731
AGGCGCC126100.08.9190511
TGGCAAA275950.08.8307071
TGGGGGC280900.08.6268531
ACGGGGT128400.08.51304151
Produced by FastQC (version 0.11.5)