FastQCFastQC Report
Thu 8 Feb 2018
YD19_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD19_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43817702
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG119400.011.5653911
TGGCCGG108600.010.9080411
TGGGGCG74250.010.6055181
TAGGCCG26550.09.9724691
AGGCCGG138900.09.8595751
AGGGGCG80150.09.8533021
TGGGGTT305950.09.8252721
TGGGCCG71250.09.7706671
AGGGCGG88100.09.71548751
AGGGCCG74350.09.4860791
ACGGGGT119700.09.4198071
AGGGTTT265650.09.4083521
TGGCCAG550800.09.0504041
TAGGCGC18200.09.0296381
TGGGGCC279650.08.9455071
GGCGTGA217450.08.8957952
GCGTGAG138650.08.8252043
TGGGCGT112050.08.7795981
TAGCCAG302950.08.7698331
TGGCGCG89950.08.75441551
Produced by FastQC (version 0.11.5)