FastQCFastQC Report
Thu 8 Feb 2018
YD20_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD20_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48446317
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG116000.011.6803881
TAGGCCG27150.09.5492391
TGGGGTT316600.09.5062911
TGGCCGG92700.09.0956261
AGGGCGA40150.08.9279621
TAGCCCG17700.08.9159081
TAGCCAG313900.08.8123961
AGGCCGG125200.08.7332961
AGGGTTT296550.08.7121931
AGGGCGG73200.08.6235831
AGGGGCG63450.08.59853651
TGGGCCG59650.08.5037971
TGGCCAG552000.08.4705281
GGGGTTT357350.08.3295342
TAGCCGA17900.08.31251
GCGGATC84200.08.28329928
AGGGGGT147900.08.2769911
TGGGGGC264050.08.0683811
CGGATCA85950.08.06229229
TGGGGCA258650.08.0450731
Produced by FastQC (version 0.11.5)