FastQCFastQC Report
Thu 8 Feb 2018
YD5_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD5_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50112278
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28850.019.1753961
CTCGTAT29800.018.9510242
CGTATGC33100.016.7217744
TCTCGTA33400.016.70626841
ATCGGAA35050.015.9186342
TCGTATG35200.015.72416443
ATCTCGT46050.012.2636140
ACACGTC48300.012.25041513
TCGGAAG50000.011.51892953
CGTCTGA53100.011.05829516
TAGCCGG142750.010.8036041
CGGAAGA56400.010.5708274
ACGGGGT127400.010.3683711
TGGCCGG96650.09.04134751
TGGGGTT307200.09.0114231
ACGTCTG68650.08.88118515
TAGGCCG27550.08.8516911
GTATGCC66850.08.85163245
CGGGGTT138400.08.7943422
TCCCGTT74550.08.6927731
Produced by FastQC (version 0.11.5)