FastQCFastQC Report
Thu 8 Feb 2018
YD6_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD6_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69460436
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG196750.012.9081631
TGGGGTT491350.010.6815391
TGGCCGG164200.010.6773071
TAGGCCG44800.010.6638291
AGGGGCG112650.010.0021671
AGGGCCG100300.09.9530941
TGGCGAA78650.09.9136291
AGGCCGG217700.09.90623951
TAGCCGA27300.09.9054431
TGGGCCG100300.09.8856921
TGGCCAG880350.09.8499721
AGGGTTT433200.09.83171
TAGCCCG29050.09.7741661
AGGGCGG122850.09.4468581
GGGGTTT568800.09.4101632
TGGGGCG105850.09.303491
TAGCCAG525350.09.0851591
TAGGCGC26050.09.0831581
GCGTGAG203200.09.0239393
ACGGGGT185150.08.9579651
Produced by FastQC (version 0.11.5)