FastQCFastQC Report
Thu 8 Feb 2018
YD7_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD7_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61962969
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG182400.012.2304441
TAGGCCG37850.010.6566081
TGGCCGG129050.010.1624121
TGGGGTT416900.010.1290691
ACGGGGT169250.09.5726471
TGGGCCG76400.09.3202071
TGGCGAA67100.09.2351081
AGGGTTT378750.09.19791
AGGGGCG74400.09.1770171
GGGGTTT485350.09.1517952
AGGCCGG168200.09.0965281
TGGCCAG766000.09.0046411
AGGGCCG74650.08.9651681
TAGCCAG495450.08.8279031
TAGCCCG22950.08.7385441
CGGGCGT110750.08.5739895
TGGGGCG72650.08.5606211
AGGGCGG86300.08.4599161
TGGCAAA364500.08.4323511
CGGGGTT180350.08.1971592