FastQCFastQC Report
Thu 8 Feb 2018
YD8_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD8_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47295401
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG133950.011.77524951
TAGGCCG29850.011.70043851
TGGGCCG74000.010.62694551
TGGGGTT327500.010.1483511
TGGCCGG116750.09.6693131
AGGGGCG84350.09.6435491
AGGGCCG76600.09.5896681
AGGCCGG152950.09.5022171
AGGGTTT293900.09.445521
TGGCCAG592000.09.1920031
ACGGGGT131350.09.0062491
GGGGTTT375350.08.9023882
TGGGGCG76900.08.8490251
AGGCGCC140350.08.7819241
TGGCGAA54550.08.7157041
AGGGGGG110600.08.6586181
GCGTGAG136800.08.6190953
TAGCCCG19900.08.6054841
GCGGATC105350.08.58505828
AGGGCGG87550.08.5446981
Produced by FastQC (version 0.11.5)