FastQCFastQC Report
Thu 8 Feb 2018
YD9_k4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameYD9_k4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57236431
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG163700.012.4040711
TGGGGTT375600.010.5662751
TAGCCGA21550.010.2486551
CGGCTAA101950.010.2176320
ACGCCCG55600.010.11629915
AGGCGCC118650.09.9529461
CCGGCTA106850.09.83328819
TAGGCCG34850.09.7647761
TGGCCAG681150.09.720691
GGGGTTT439650.09.6834572
TGGCCGG111950.09.6829811
TAGCCAG445650.09.5628151
AGGGTTT354400.09.3923641
CGCCCGG94450.09.290071516
ACGGGGT140550.09.25194551
TGGCGAA55800.09.1681091
TAGGCGC19350.09.0845151
TGGGCCG65750.09.048911
TGGGCGA70900.08.9637761
AGGGGCG65050.08.765191
Produced by FastQC (version 0.11.5)