FastQCFastQC Report
Thu 8 Feb 2018
OD10_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD10_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42610619
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG28400.011.5196791
TAGCCGG102150.010.8671941
TGGGCCG67300.010.7616951
AGGGCGG87300.010.4672231
TGGCCGG100200.010.0879061
AGGCCGG138600.09.9627421
TGGGGCG72650.09.8760251
AGGCGCC128800.09.8448961
TAGCCCG17600.09.7429751
AGGGGCG86200.09.6846781
TCGTATA31150.09.67753112
ACTAGAC106450.09.49008422
AGGGCCG69900.09.4253251
TGGGGTT262800.09.4097021
CTTCGTA40000.09.3361110
TGGCCAG451750.09.3247421
AGGCGAC27850.09.3167271
TGGGGCC224500.09.0753231
TCCCGTT88450.09.0556821
TAGGCGT20700.09.0468721
Produced by FastQC (version 0.11.5)