FastQCFastQC Report
Thu 8 Feb 2018
OD11_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD11_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44658687
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG28050.011.4006231
TAGCCGG104500.011.2277971
TGGGGCG65400.09.9516061
TCGTATA29150.09.94794512
AGGGGCG82800.09.8186081
TGGCCGG98250.09.76450351
TAGCCCG17100.09.7456041
AGGGCGC58950.09.397761
TCCCGTT94550.09.1938811
TGGGCCG63200.09.193391
TGGGGTT263300.09.1090241
AGGCCGG127950.08.9588161
AGGGCCG66350.08.9266381
GCGTGAG112350.08.924193
TGGCGAT33850.08.8484271
TGGCCAG460250.08.8417341
AGGCGGC83500.08.7923351
TAGCCGA16650.08.7916871
TAGCCAG298450.08.7304141
AGGGTTT259500.08.73039151
Produced by FastQC (version 0.11.5)