FastQCFastQC Report
Thu 8 Feb 2018
OD12_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD12_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49108323
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14700.017.44080441
CTCGTAT19150.013.3006937
CGTATGC20700.012.49392739
TCTCGTA20900.011.86344636
TAGCCGG128750.011.5151921
TGGGCCG66600.010.4876941
TCGTATG24050.010.47368738
TAGGCCG31100.010.2153031
TCCCGTT102750.010.1748661
TGGCCGG103600.09.4824221
TGGGGTT303750.09.3983771
ATGCCGT28050.09.38063942
AGGGCCG69350.09.1296141
TGGCCAG533050.09.1132521
TGGCGAA46200.09.0711741
AGGGCGG81600.08.9739941
TAGCCCG18850.08.9648381
AGGGTTT281500.08.9166011
GGGGTTT341950.08.8477512
CCAACGA107100.08.81854259
Produced by FastQC (version 0.11.5)