FastQCFastQC Report
Thu 8 Feb 2018
OD13_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD13_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43094680
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG110550.012.0555741
TGGGCCG81050.011.3796651
AGGGGCG108700.010.8915311
TGGCCGG106350.010.77172851
TAGGCCG29900.010.5588351
AGGGCCG86300.010.2693021
AGGCGCC128700.010.0225131
TGGGGCG84750.09.898341
AGGGCGG103750.09.6071221
TGGGGTT262650.09.4100581
AGGGCGA50300.09.3699541
AGGGCGC72500.09.1757841
AGGCCGG147050.09.1552131
TGGGCGG102800.09.1255571
TGGCGGC95300.09.1101811
TAGCCCG21550.09.1039771
TGGGGCC227500.09.0400871
TGGCCAG441100.09.0131051
AGGGGGG102100.08.9893411
AGGCGCG75100.08.9782231
Produced by FastQC (version 0.11.5)