FastQCFastQC Report
Thu 8 Feb 2018
OD14_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD14_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39803554
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG94900.011.7703171
TGGGGCG41350.010.0755541
AGGGCCG40450.09.2975961
TAGGCCG22650.09.2467161
AGCGTCG6450.09.077921
AGGGGCG45850.08.9393251
TGGGCCG38750.08.7756221
TAGGCGG16100.08.6723841
TGGGGTT229700.08.5786671
TGGCCGG65500.08.2516851
ATCTTCG42800.08.0877838
AGGCCGG88300.08.0848381
TACTCGG111750.08.08477737
TAGGCGA11200.08.0429371
TCGTATA28250.08.03668112
TAGCCAG273500.07.9458931
TGGCCAG380100.07.9155541
AGGGCGG47300.07.90350341
AGGGTTT229400.07.90269371
TGGCGAA33500.07.86527251
Produced by FastQC (version 0.11.5)