FastQCFastQC Report
Thu 8 Feb 2018
OD15_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD15_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40959327
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG113050.013.5304421
TAGCCGA13750.010.5009871
TAGGCCG26800.09.933451
AGGGTTT244850.09.8406541
TGGGGCG58050.09.8326631
TGGGCCG54800.09.7982661
TGGGGTT249000.09.7672491
TCCCGTT69850.09.5927451
TAGCCAG312350.09.5053311
AGGGCCG58400.09.3874211
TAGGCGC14850.09.26736451
TGGCCGG79050.09.0185781
AGGGCGG69200.08.9656021
AGGGGCG66800.08.9499851
GGGGTTT289000.08.9441072
GTAGTCC243700.08.81007226
CCAACGA75850.08.8087789
TACTCGG139250.08.75656337
AGGGTCG26300.08.7497831
TCCAACG79350.08.59034258
Produced by FastQC (version 0.11.5)