FastQCFastQC Report
Thu 8 Feb 2018
OD16_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD16_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43918548
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG27050.011.7604421
TAGCCGG110300.011.5978991
AGGGCCG58850.09.6994211
TGGGGTT261700.09.6988581
TCGTATA26050.09.58605212
TGGCCGG85050.09.496871
TGGGCCG60800.09.4625541
AGGCCGG118500.09.19603351
AGGGGCG69550.09.1479491
TGGGGCG58250.08.9859471
AGGGCGG71450.08.967841
AGGGTTT258650.08.9671091
TAGGGCG12950.08.8853021
GGGGTTT304750.08.6296932
AGGGGGG80650.08.5323231
TCCCGTT84800.08.4872431
TAGCCAG315700.08.4186511
TAGCCCG16650.08.4013531
TGGCGGC64850.08.3149511
CTTCGTA34200.08.22258710
Produced by FastQC (version 0.11.5)