FastQCFastQC Report
Thu 8 Feb 2018
OD17_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD17_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51832594
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG137800.013.0418071
TGGGCCG62300.010.4239681
TAGGCCG30950.010.2727591
TGGGGTT323250.09.8776451
TGGCCGG100250.09.8293711
TAGCCGA19350.09.7887441
AGGGGCG69650.09.4210771
AGGGTTT306200.09.3819451
AGGCGCC115300.09.1526161
TCCCGTT98550.09.1523381
TGGCCAG565050.09.1066241
GGGGTTT379000.09.0277712
TAGCCAG382600.08.9288651
GCGTGAG125050.08.90455153
AGGCCGG131500.08.7967091
AGGGCCG64600.08.7613271
CGTGAGC124600.08.7381174
AGGGCGG69300.08.6551991
TGGGGCC241100.08.6511371
TGGGGCG60750.08.6113331
Produced by FastQC (version 0.11.5)