FastQCFastQC Report
Thu 8 Feb 2018
OD18_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD18_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28131765
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG79850.013.176491
TCCCGTT44400.09.8948551
TAGCCAG229550.09.7460751
AGGGTTT173100.09.5143281
TGGCGAA23450.09.4154481
TGGGGTT175300.09.38207151
CGGGCGT46700.09.2975865
GTAGTCC171500.08.76354726
TAGCCCG10900.08.6812161
TAGCCGA10400.08.6653161
AGGGCCG35750.08.6338061
CCAACGA45700.08.6150199
CCCGTTT56500.08.6007252
TACTCGG99550.08.59001837
GGCGAAA23350.08.4786282
GGGGTTT209550.08.4492242
TGGGCCG31900.08.4045421
TGGCCAG319850.08.3822071
TCCAACG47300.08.3235138
CTACTCG104050.08.30484736