FastQCFastQC Report
Thu 8 Feb 2018
OD19_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD19_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20233347
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG55900.012.5749911
TGGGGTT128650.09.6670551
AGGGGCG35250.09.2038231
TAGCCCG7500.09.0120761
TGGCGAA18250.08.8886251
TAGCCAG150200.08.8650751
GGGGTTT147000.08.7692852
AGGGTTT116250.08.5856991
TGGGGCG32100.08.5628771
TAGCGTC5851.8007995E-108.4728941
AGGGCGG33800.08.4654871
TGGCCGG45250.08.4643821
AGGCCCG27350.08.237731
AGGGCGC25500.08.2168931
TCCCGTT33500.08.1377711
AGGGCCG29950.08.124411
TAGGCCG11650.08.1224731
TGGGTCG10900.08.0612621
TGGCCAG225550.08.0611241
CGGATCA28300.08.02980129
Produced by FastQC (version 0.11.5)