FastQCFastQC Report
Thu 8 Feb 2018
OD1_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD1_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52305782
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG103700.011.9392861
TGGGGTT296250.010.0408681
AGGGGCG75900.010.01321
TAGGCCG27850.09.798171
AGGGCCG58650.09.7283081
TGGGCCG60950.09.5832061
TGGGGCG62350.09.5488771
AGGCGCC103200.09.1999861
TGGCCGG89700.09.0761051
AGGGTTT300100.09.0553631
TCCCGTT95450.08.9782661
AGGGCGG71500.08.9577121
GCGTGAG114900.08.67373
AGGCCGG114800.08.6632621
AGGGGGC157300.08.5878191
GGGGTTT339600.08.585352
CGTGAGC113650.08.47217754
TGGCGAA44100.08.3866811
GGCGTGA182400.08.32529452
TGGGCGG81050.08.2639611
Produced by FastQC (version 0.11.5)