FastQCFastQC Report
Thu 8 Feb 2018
OD20_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD20_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33252165
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG77900.011.77021
TATGCCG10600.011.67570341
AGGGGCG90650.011.1087861
TGGGCCG68600.010.8700291
CTCGTAT10850.010.78483637
CGTATGC11500.010.76198539
TGGGGTT211900.010.2487971
TGGCCGG88850.010.2435571
TAGGCGT17600.010.2400991
TGGGGCG76700.010.1920371
AGGGCGG91250.010.1222681
TAGCCCG14750.09.9276891
TCTCGTA13050.09.65634836
AGGGCCG73300.09.4661541
AGGGTTT195100.09.4570021
TGGCCCG53000.09.3513361
AGGCGGC95700.09.27494051
TGGGGCC187950.09.253411
TGGGCGG85700.09.2531311
TAGCCAG213500.09.1801171
Produced by FastQC (version 0.11.5)