FastQCFastQC Report
Thu 8 Feb 2018
OD2_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD2_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52296404
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG117200.011.2568511
TGGGGTT316150.09.3946641
TGGGGCG65650.09.103321
AGGGCCG66050.09.0823351
AGGGGCG77850.09.0672251
TCCCGTT92900.08.9092071
TAGGCCG27800.08.8424041
AGGCGAC26250.08.7631361
AGGCCGG128250.08.7395231
AGGCGCG52900.08.7395061
TGGGCCG64050.08.732151
AGGGTTT301600.08.6888731
TGGCGAA44700.08.5768951
AGGCGCC117650.08.5301511
TAGCCCG16900.08.4070291
AGGGCGG76450.08.3777921
TGGCCGG96600.08.357851
GGGGTTT358750.08.3526652
AGGGGGT135800.08.2370291
TGGGGGG185150.08.2218341
Produced by FastQC (version 0.11.5)