FastQCFastQC Report
Thu 8 Feb 2018
OD3_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD3_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30633007
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG59600.010.2531741
AGGGCGC60950.09.6561071
TGGGGCG68250.09.6144751
AGGGCCG62750.09.3072471
AGGGGCG85550.09.3044331
TGGGGTT182550.09.2643441
TGGCGGC77450.09.2585071
TGGGCGG78600.09.2091121
TGGGCCG58350.09.1588881
AGGGCGG84150.09.0572811
AGGCGCC89450.08.9995981
TCCCGTT52900.08.9941891
AGGCGCG60750.08.8712911
AGGGTTT166450.08.8057181
CTCGTAT11800.08.76931542
AGGGGGG72800.08.6109031
AGGGTCG30950.08.5243331
TGGCCGG73050.08.457961
TAGCCGA13150.08.4024431
AGGGGGC133450.08.3810481
Produced by FastQC (version 0.11.5)