FastQCFastQC Report
Thu 8 Feb 2018
OD4_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD4_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55064963
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG116600.010.4400781
TAGGGCG19200.09.97991
TGGGGCG92550.09.9134881
TAGGCCG32100.09.4806311
AGGGCGG104750.09.2969011
TGGGGTT319200.09.1174181
AGGGGCG109950.09.0212341
TGGGCCG78950.08.7373111
TGGCCGG110400.08.6169161
AGGCGCC131200.08.60821
TAGGCGC20600.08.4262051
AGGGCCG86300.08.3849941
AGGGGGG111050.08.2010851
GGGGTTT347450.08.1758362
TGGCGAA48550.08.1720671
AGGGGGC198700.08.111791
AGGGACG61700.08.1110351
TGGCCAG511050.08.0502241
TCCCGTT86450.07.97930241
TGGGGGC265300.07.9617921
Produced by FastQC (version 0.11.5)