FastQCFastQC Report
Thu 8 Feb 2018
OD5_k4me3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOD5_k4me3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61167212
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG147100.011.9884131
TAGGCCG32650.010.0841041
TGGGGTT363400.09.8172341
AGGGGCG87400.09.05656051
TGGGCCG70700.08.9311271
GGGGTTT414400.08.7236042
TCCCGTT96450.08.7211591
AGGGCGG89950.08.5240381
AGGGTTT366050.08.4647461
TGGGGCG76650.08.4143681
TAGCCAG431700.08.4102991
TAGCCGA22500.08.3188491
TGGCGGC81000.08.2687351
TAGGCGA17350.08.1885821
TGGCGTT37750.08.06463051
CGGGCGT89800.07.9909795
TAGGCGC20050.07.9856741
CGGCTAA88600.07.947554620
GCGTGAG127350.07.93109273
AGGCGCC125850.07.90229031
Produced by FastQC (version 0.11.5)